RADAR | Search for sensor designs | Human genes | Mouse genes | Code

Pregenerated sensors for GSTM4 (Mus musculus)

Ensembl gene IDs: ENSMUSG00000027890

Ensembl transcript IDs: ENSMUST00000029489.15, ENSMUST00000106670.2, ENSMUST00000178808.8

Found 33 sensor candidate(s). Results are sorted by "preference number" (better first), and then by GC (higher first).

Type Sensor Details
3UTR TCA 90 bp CGTCTCTGGTCTCCAGACAAGCATGGGGAGCCCCAGGCTGAGTGCTaGTGGAGAGTCCCAACTCTTCACAACAGCACAGCTGCTGGCAGG +
Preference number: 1 (lower is better)
Translates to: RLWSPDKHGEPQAEC*WRVPTLHNSTAAGR
Trigger: CCTGCCAGCAGCTGTGCTGTTGTGAAGAGTTGGGACTCTCCATCAGCACTCAGCCTGGGGCTCCCCATGCTTGTCTGGAGACCAGAGACG
60% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR CCA 90 bp TGGGCAGAGAGGATCCTGGCTTTCTGCTGGGGACTCCTATCAGCCTaGATCCTCACCACTGAGACCTGCATGACAAATCTCAGTGCTGCT +
Preference number: 1 (lower is better)
Translates to: WAERILAFCWGLLSA*ILTTETCMTNLSAA
Trigger: AGCAGCACTGAGATTTGTCATGCAGGTCTCAGTGGTGAGGATCCAGGCTGATAGGAGTCCCCAGCAGAAAGCCAGGATCCTCTCTGCCCA
56% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR CCA 90 bp GACAGGATCCTGGAGACATATAGATATAAGGCAGCCATAGCACAGTaGGCAGAGAGGATCCTGGCTTTCTGCTGGGGACTCCTATCAGCC +
Preference number: 1 (lower is better)
Translates to: DRILETYRYKAAIAQ*AERILAFCWGLLSA
Trigger: GGCTGATAGGAGTCCCCAGCAGAAAGCCAGGATCCTCTCTGCCCACTGTGCTATGGCTGCCTTATATCTATATGTCTCCAGGATCCTGTC
53% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR CCA 90 bp CACACCAGCCGTCTCTGGTCTCCAGACAAGCATGGGGAGCCCCAGGCTGAGTGCGGACCTTTGACACTCTaGGGTCAGGAAGGGGATGGA +
Preference number: 2 (lower is better)
Translates to: HTSRLWSPDKHGEPQAECGPLTL*GQEGDG
Trigger: TCCATCCCCTTCCTGACCCCAGAGTGTCAAAGGTCC...GCACTCAGCCTGGGGCTCCCCATGCTTGTCTGGAGACCAGAGACGGCTGGTGTG
61% GC, longest homopolymer 4 nt, total homopolymer stretches 12 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR TCA 90 bp GGAGGGACCCAAAAGAGCTGTGCAGAGACCAAATTCACACACCAGCCGTCTCTGTGGGGAGCCCCAGGCTaGGTGCTGATGGAGAGTCCC +
Preference number: 2 (lower is better)
Translates to: GGTQKSCAETKFTHQPSLWGAPG*VLMESP
Trigger: GGGACTCTCCATCAGCACTCAGCCTGGGGCTCCCCA...CAGAGACGGCTGGTGTGTGAATTTGGTCTCTGCACAGCTCTTTTGGGTCCCTCC
60% GC, longest homopolymer 4 nt, total homopolymer stretches 12 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR CCA 90 bp GCCTTCTGCAGCTGCCTCCTGAGAAACAAACAGGTGGGGCTAGGAAGGGCCAAACACACCAGCCGTCTCTaGTCTCCAGACAAGCATGGG +
Preference number: 2 (lower is better)
Translates to: AFCSCLLRNKQVGLGRAKHTSRL*SPDKHG
Trigger: CCCATGCTTGTCTGGAGACCAGAGACGGCTGGTGTG...TTTGGCCCTTCCTAGCCCCACCTGTTTGTTTCTCAGGAGGCAGCTGCAGAAGGC
58% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR TCA 90 bp GCCTTCTGCAGCTGCCTCCTGAGAAACAAACAGGTGGGGCTAGGAAGGGCCAAACAACTAAAATCCCTGTaGACTCAGCCTCTGCCCGGG +
Preference number: 2 (lower is better)
Translates to: AFCSCLLRNKQVGLGRAKQLKSL*TQPLPG
Trigger: CCCGGGCAGAGGCTGAGTTCACAGGGATTTTAGTTG...TTTGGCCCTTCCTAGCCCCACCTGTTTGTTTCTCAGGAGGCAGCTGCAGAAGGC
57% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR GCA 90 bp GCCTTCTGCAGCTGCCTCCTGAGAAACAAACAGGTGGGGCTAGGAAGGGCCAAATCACAACAGCACAGCTaGTGGCAGGCAAGACCATCA +
Preference number: 2 (lower is better)
Translates to: AFCSCLLRNKQVGLGRAKSQQHS*WQARPS
Trigger: TGATGGTCTTGCCTGCCAGCAGCTGTGCTGTTGTGA...TTTGGCCCTTCCTAGCCCCACCTGTTTGTTTCTCAGGAGGCAGCTGCAGAAGGC
56% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR CCA 90 bp CCTGCCTATCCAACTAAAATCCCTGTGAACTCAGCCTCTGCCCGGGTCACTAGGCAGCCAGAGCCAGTGTaGTTCAGGCTTTACAGGAGG +
Preference number: 2 (lower is better)
Translates to: PAYPTKIPVNSASARVTRQPEPV*FRLYRR
Trigger: CCTCCTGTAAAGCCTGAACCACACTGGCTCTGGCTG...CCTAGTGACCCGGGCAGAGGCTGAGTTCACAGGGATTTTAGTTGGATAGGCAGG
56% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR GCA 90 bp CAGGAGGGACCCAAAAGAGCTGTGCAGAGACCAAATTCACACACCAGCCGTCTCTCACAACAGCACAGCTaGTGGCAGGCAAGACCATCA +
Preference number: 2 (lower is better)
Translates to: QEGPKRAVQRPNSHTSRLSQQHS*WQARPS
Trigger: TGATGGTCTTGCCTGCCAGCAGCTGTGCTGTTGTGA...GAGACGGCTGGTGTGTGAATTTGGTCTCTGCACAGCTCTTTTGGGTCCCTCCTG
54% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR CCA 90 bp CCTGCCTATCCAACTAAAATCCCTGTGAACTCAGCCTCTGCCCGGGTCACTAGGCAACAGCACAGCTGCTaGCAGGCAAGACCATCAAGT +
Preference number: 2 (lower is better)
Translates to: PAYPTKIPVNSASARVTRQQHSC*QARPSS
Trigger: ACTTGATGGTCTTGCCTGCCAGCAGCTGTGCTGTTG...CCTAGTGACCCGGGCAGAGGCTGAGTTCACAGGGATTTTAGTTGGATAGGCAGG
54% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR TCA 90 bp GGAGGGACCCAAAAGAGCTGTGCAGAGACCAAATTCACACACCAGCCGTCTCTGAGGGCAGGGACTGGATaGAAGAAAAGAGGACCTTTG +
Preference number: 2 (lower is better)
Translates to: GGTQKSCAETKFTHQPSLRAGTG*KKRGPL
Trigger: CAAAGGTCCTCTTTTCTTTCATCCAGTCCCTGCCCT...CAGAGACGGCTGGTGTGTGAATTTGGTCTCTGCACAGCTCTTTTGGGTCCCTCC
53% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR CCA 72 bp GCTGTGCAGAGACCAAATTCACACACCAGCCGTCTCTaGTCTCCAGACAAGCATGGGGAGCCCCAGGCTGAG +
Preference number: 3 (lower is better)
Translates to: AVQRPNSHTSRL*SPDKHGEPQAE
Trigger: CTCAGCCTGGGGCTCCCCATGCTTGTCTGGAGACCAGAGACGGCTGGTGTGTGAATTTGGTCTCTGCACAGC
60% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR TCA 72 bp AGGGCCAAACCCCTGCCTATCCAACTAAAATCCCTGTaGACTCAGCCTCTGCCCGGGTCACTAGGCAGCCAC +
Preference number: 3 (lower is better)
Translates to: RAKPLPIQLKSL*TQPLPGSLGSH
Trigger: GTGGCTGCCTAGTGACCCGGGCAGAGGCTGAGTTCACAGGGATTTTAGTTGGATAGGCAGGGGTTTGGCCCT
58% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR GCA 72 bp CTGATGGAGAGTCCCAACTCTTCACAACAGCACAGCTaGTGGCAGGCAAGACCATCAAGTGGGCGCGGGACA +
Preference number: 3 (lower is better)
Translates to: LMESPNSSQQHS*WQARPSSGRGT
Trigger: TGTCCCGCGCCCACTTGATGGTCTTGCCTGCCAGCAGCTGTGCTGTTGTGAAGAGTTGGGACTCTCCATCAG
58% GC, longest homopolymer 3 nt, total homopolymer stretches 0 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR CCA 72 bp ATGGAGAGTCCCAACTCTTCACAACAGCACAGCTGCTaGCAGGCAAGACCATCAAGTGGGCGCGGGACACAG +
Preference number: 3 (lower is better)
Translates to: MESPNSSQQHSC*QARPSSGRGTQ
Trigger: CTGTGTCCCGCGCCCACTTGATGGTCTTGCCTGCCAGCAGCTGTGCTGTTGTGAAGAGTTGGGACTCTCCAT
58% GC, longest homopolymer 3 nt, total homopolymer stretches 0 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR CCA 72 bp AGGATCCTGGCTTTCTGCTGGGGACTCCTATCAGCCTaGATCCTCACCACTGAGACCTGCATGACAAATCTC +
Preference number: 3 (lower is better)
Translates to: RILAFCWGLLSA*ILTTETCMTNL
Trigger: GAGATTTGTCATGCAGGTCTCAGTGGTGAGGATCCAGGCTGATAGGAGTCCCCAGCAGAAAGCCAGGATCCT
54% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR TCA 72 bp AGAGGAGGGATTAACCAAGATGGCCCCCATCCCTGGTaGCCAAGACGGGATACTCTGAAGACATCAGAGACA +
Preference number: 3 (lower is better)
Translates to: RGGINQDGPHPW*PRRDTLKTSET
Trigger: TGTCTCTGATGTCTTCAGAGTATCCCGTCTTGGTCACCAGGGATGGGGGCCATCTTGGTTAATCCCTCCTCT
53% GC, longest homopolymer 5 nt, total homopolymer stretches 5 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR-3UTR CAA 90 bp CCTGCCTATCCAACTAAAATCCCTGTGAACTCAGCCTCTGCCCGGGTCACTAGGAAGAAAAGAGGACCTTaGACACTCTGGGGTCAGGAA +
Preference number: 3 (lower is better)
Translates to: PAYPTKIPVNSASARVTRKKRGP*TLWGQE
Trigger: TTCCTGACCCCAGAGTGTCAAAGGTCCTCTTTTCTT...CCTAGTGACCCGGGCAGAGGCTGAGTTCACAGGGATTTTAGTTGGATAGGCAGG
52% GC, longest homopolymer 4 nt, total homopolymer stretches 12 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
3UTR GCA 72 bp GGACTCCTATCAGCCTGGATCCTCACCACTGAGACCTaGATGACAAATCTCAGTGCTGCTAGCATAAAGGAG +
Preference number: 3 (lower is better)
Translates to: GLLSAWILTTET*MTNLSAASIKE
Trigger: CTCCTTTATGCTAGCAGCACTGAGATTTGTCATGCAGGTCTCAGTGGTGAGGATCCAGGCTGATAGGAGTCC
51% GC, longest homopolymer 3 nt, total homopolymer stretches 0 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp CAGGAAGTTCAACTGTTCCCAGAACCCCCGGTTCCCACTTCCTGCCCAGTCAAGACAAACCATGTCCGCTaGCCCAGGAACTGTGAGAAG +
Preference number: 4 (lower is better)
Translates to: QEVQLFPEPPVPTSCPVKTNHVR*PRNCEK
Trigger: CTTCTCACAGTTCCTGGGCCAGCGGACATGGTTTGT...CTTGACTGGGCAGGAAGTGGGAACCGGGGGTTCTGGGAACAGTTGAACTTCCTG
56% GC, longest homopolymer 5 nt, total homopolymer stretches 5 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8
CDS-3UTR TCA 90 bp CAGGAAGTTCAACTGTTCCCAGAACCCCCGGTTCCCACTTCCTGCCCAGTCAAGTGGCATTGCTCTGCGTaGTCTTGTGTGACCCATCAA +
Preference number: 4 (lower is better)
Translates to: QEVQLFPEPPVPTSCPVKWHCSA*SCVTHQ
Trigger: TTGATGGGTCACACAAGATCACGCAGAGCAATGCCA...CTTGACTGGGCAGGAAGTGGGAACCGGGGGTTCTGGGAACAGTTGAACTTCCTG
54% GC, longest homopolymer 5 nt, total homopolymer stretches 5 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp CAGGAAGTTCAACTGTTCCCAGAACCCCCGGTTCCCACTTCCTGCCCAGTCAAGCAGGAGCGTCTCCCATaGTGTATCTCTTCTCTTCAT +
Preference number: 4 (lower is better)
Translates to: QEVQLFPEPPVPTSCPVKQERLP*CISSLH
Trigger: ATGAAGAGAAGAGATACACCATGGGAGACGCTCCTG...CTTGACTGGGCAGGAAGTGGGAACCGGGGGTTCTGGGAACAGTTGAACTTCCTG
53% GC, longest homopolymer 5 nt, total homopolymer stretches 5 nt.
Matches the following 1 transcripts:
ENSMUST00000029489.15
CDS-3UTR CCA 90 bp GAGAGGATCCTGGCTTTCTGCTGGGGACTCCTATCAGCCTGGATCCTCACCACTCAGTTTCTCAAAGTCTaGGCTGTAACAGACTCGAGC +
Preference number: 4 (lower is better)
Translates to: ERILAFCWGLLSAWILTTQFLKV*AVTDSS
Trigger: GCTCGAGTCTGTTACAGCCCAGACTTTGAGAAACTG...AGTGGTGAGGATCCAGGCTGATAGGAGTCCCCAGCAGAAAGCCAGGATCCTCTC
53% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
CDS-3UTR TCA 90 bp TGGGGTCAGGAAGGGGATGGACAGAAAGCAGCACTAGGGCTACAGGAAGTTCAATGGCATTGCTCTGCGTaGTCTTGTGTGACCCATCAA +
Preference number: 4 (lower is better)
Translates to: WGQEGDGQKAALGLQEVQWHCSA*SCVTHQ
Trigger: TTGATGGGTCACACAAGATCACGCAGAGCAATGCCA...TTGAACTTCCTGTAGCCCTAGTGCTGCTTTCTGTCCATCCCCTTCCTGACCCCA
52% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp GAAGTTCAACTGTTCCCAGAACCCCCGGTTCCCACTTCCTGCCCAGTCAAGGCTACTTATTGCCCCAAGTaGCCACCTTTGTATATAGGG +
Preference number: 4 (lower is better)
Translates to: EVQLFPEPPVPTSCPVKATYCPK*PPLYIG
Trigger: CCCTATATACAAAGGTGGCCACTTGGGGCAATAAGT...AGCCTTGACTGGGCAGGAAGTGGGAACCGGGGGTTCTGGGAACAGTTGAACTTC
52% GC, longest homopolymer 5 nt, total homopolymer stretches 9 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp GACCCAAAAGAGCTGTGCAGAGACCAAATTCACACACCAGCCGTCTCTGGTCTCCAGTTTCTCAAAGTCTaGGCTGTAACAGACTCGAGC +
Preference number: 4 (lower is better)
Translates to: DPKELCRDQIHTPAVSGLQFLKV*AVTDSS
Trigger: GCTCGAGTCTGTTACAGCCCAGACTTTGAGAAACTG...GAGACCAGAGACGGCTGGTGTGTGAATTTGGTCTCTGCACAGCTCTTTTGGGTC
51% GC, longest homopolymer 4 nt, total homopolymer stretches 4 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp TGGGGTCAGGAAGGGGATGGACAGAAAGCAGCACTAGGGCTACAGGAAGTTCAACAGTTTCTCAAAGTCTaGGCTGTAACAGACTCGAGC +
Preference number: 4 (lower is better)
Translates to: WGQEGDGQKAALGLQEVQQFLKV*AVTDSS
Trigger: GCTCGAGTCTGTTACAGCCCAGACTTTGAGAAACTG...TTGAACTTCCTGTAGCCCTAGTGCTGCTTTCTGTCCATCCCCTTCCTGACCCCA
51% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp CCTGAGAAACAAACAGGTGGGGCTAGGAAGGGCCAAACCCCTGCCTATCCAACTACTTATTGCCCCAAGTaGCCACCTTTGTATATAGGG +
Preference number: 4 (lower is better)
Translates to: PEKQTGGARKGQTPAYPTTYCPK*PPLYIG
Trigger: CCCTATATACAAAGGTGGCCACTTGGGGCAATAAGT...AGTTGGATAGGCAGGGGTTTGGCCCTTCCTAGCCCCACCTGTTTGTTTCTCAGG
51% GC, longest homopolymer 4 nt, total homopolymer stretches 12 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
CDS-3UTR CCA 90 bp GCGCGGGACACAGTCATGCTAGAAGGGCATTTTGCTAAAGGAAAACTAGGAGCCACTTATTGCCCCAAGTaGCCACCTTTGTATATAGGG +
Preference number: 4 (lower is better)
Translates to: ARDTVMLEGHFAKGKLGATYCPK*PPLYIG
Trigger: CCCTATATACAAAGGTGGCCACTTGGGGCAATAAGT...GGCTCCTAGTTTTCCTTTAGCAAAATGCCCTTCTAGCATGACTGTGTCCCGCGC
49% GC, longest homopolymer 4 nt, total homopolymer stretches 12 nt.
Matches the following 2 transcripts:
ENSMUST00000029489.15
ENSMUST00000178808.8
CDS-3UTR CAA 90 bp AGGAAGTTCAACTGTTCCCAGAACCCCCGGTTCCCACTTCCTGCCCAGTCAAGGTCTTCTCTTCATAGCTaGAGCCTGTGTATTCTAGGA +
Preference number: 5 (lower is better)
Translates to: RKFNCSQNPRFPLPAQSRSSLHS*SLCILG
Trigger: TCCTAGAATACACAGGCTCAAGCTATGAAGAGAAGA...CCTTGACTGGGCAGGAAGTGGGAACCGGGGGTTCTGGGAACAGTTGAACTTCCT
51% GC, longest homopolymer 5 nt, total homopolymer stretches 5 nt.
Matches the following 1 transcripts:
ENSMUST00000029489.15
CDS CCA 90 bp AGGAAATCTACAAAAGTAATCTTTTCACCAACAAACCATGTCCGCTaGCCCAGGAACTGTGAGAAGAGCTTCACCATTCCAGGGAGCTGC +
Preference number: 5 (lower is better)
Translates to: RKSTKVIFSPTNHVR*PRNCEKSFTIPGSC
Trigger: GCAGCTCCCTGGAATGGTGAAGCTCTTCTCACAGTTCCTGGGCCAGCGGACATGGTTTGTTGGTGAAAAGATTACTTTTGTAGATTTCCT
48% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8
CDS CCA 72 bp ACAAAAGTAATCTTTTCACCAACAAACCATGTCCGCTaGCCCAGGAACTGTGAGAAGAGCTTCACCATTCCA +
Preference number: 6 (lower is better)
Translates to: TKVIFSPTNHVR*PRNCEKSFTIP
Trigger: TGGAATGGTGAAGCTCTTCTCACAGTTCCTGGGCCAGCGGACATGGTTTGTTGGTGAAAAGATTACTTTTGT
46% GC, longest homopolymer 4 nt, total homopolymer stretches 8 nt.
Matches the following 3 transcripts:
ENSMUST00000029489.15
ENSMUST00000106670.2
ENSMUST00000178808.8